TECHNO CHEMICAL corp.

HOPE^® - Formalin-free fixation technique for molecular pathology and research!

Tissue conservation using the new HOPE^®-technique provides paraffin embedded tissue blocks with native structure of the macro-molecules (protein, DNA, RNA) in the tissue.

During HOPE^® fixation, tissue samples are incubated in HOPE^® I solution, then in HOPE^® II solution, followed by a dehydration with acetone and subsequent embedding in paraffin. For this tissue processing no alcohols or aldehydes (formalin, glutaraldehyde) are used. As a consequence, there is no cross-linking of macro- molecular structures during the process. This opens up new opportunities to characterize tissues out of paraffin blocks.

Applications for immunohistochemistry, in situ hybridization targeting RNA and DNA, PCR and RT-PCR have been demonstrated using properly prepared paraffin embedded tissue sections. Tissue specimens fixed with the HOPE^® technique allow greater morphology and retrospective studies on the molecular level.

The HOPE^®-Fixation technique requires HOPE^® Fixative System II and HOPE^® Fixative System I to complete the protocol.

Applications:

• PCR
• RT-PCR
• Immunohistochemistry
• In situ hybridization targeting RNA and DNA

Benefits:

• Achieve IHC on paraffin tissue without antigen unmasking
• IHC using antibodies which do not work on formalin fixed parafffin embedded tissue
• No more overfixed formalin tissue samples
• Unmatched results in RNA and DNA in situ hybridization
• Excellent conservation of nucleic acids for PCR and RT-PCR