TECHNO CHEMICAL corp.

When circumstances call for the rapid development of microsphere reagents, particles coated with affinity binding proteins offer a ready solution. Affinity binding protocols are straightforward, typically involving a simple incubation of the spheres with the appropriate ligand. Binding proteins also provide an opportunity to optimally orient the ligand, as with the end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein A or G. Particles coated with binding proteins also find significant use in purifications. Streptavidin-coated microspheres are often used in the isolation of biotinylated PCR amplicons. Protein A and Protein G microspheres are utilized for purification of antibodies and Fc-tagged fusion proteins, as well as for the recovery of Ab/Ag complexes in immunoprecipitation. Streptavidin-coated fluorescent microspheres supplied as ~1% solids (w/v) aqueous suspensions.: